VGO-0152
QUESTION
Bull Sperm sexing can be done by?
OPTIONS
(A) Spectrophotometer
(B) Bovine photometer
(C) Electron microscope
(D) Flow cytometer
ANSWER
(D) Flow cytometer
EXPLANATION
Flow cytometry can distinguish X-bearing and Y-bearing bovine sperm through their differing DNA content after fluorescent staining. Sorted populations can then be collected for sexed semen production. Spectrophotometers and bovine photometers mainly estimate semen concentration through optical measurements, while electron microscopy examines ultrastructure. None of those alternative instruments performs the routine high-throughput DNA-based sorting process.
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Veterinary Gynaecology and Obstetrics · 240 Questions
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