Which of the following cryoprotective are used in semen freezing? (a) Glycerol (b) Acetic acid (c) Normal saline (d) DMSO?

VGO-0164

QUESTION

Which of the following cryoprotective are used in semen freezing:

(a) Glycerol
(b) Acetic acid
(c) Normal saline
(d) DMSO

OPTIONS

(A) Only b and c
(B) Only a and c
(C) Only a and d
(D) Only b and c

ANSWER

(C) Only a and d

EXPLANATION

Glycerol and dimethyl sulfoxide are permeating cryoprotectants that reduce cellular injury during freezing and thawing. Acetic acid is not a standard sperm cryoprotectant, and normal saline alone cannot provide adequate protection against ice formation and freeze-concentration effects. Consequently, only the combination containing glycerol and DMSO correctly identifies two cryoprotective agents among the listed substances.

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