VMC-0100
QUESTION
Match the following:
| Type of Microscope | Used for Observing |
|---|---|
| (a) Bright field | (1) FISH Slide |
| (b) Electron | (2) Unstained Leptospira organisms |
| (c) Dark field | (3) Embryo |
| (d) Fluorescent | (4) Virus |
| (e) Inverted | (5) Cell division |
Match as (a), (b), (c), (d):
OPTIONS
(A) a-5; c-2; b-1; e-3; d-4
(B) b-1; d-2; e-3; c-4; a-5
(C) b-4; a-5; c-2; d-1; e-3
(D) a-1; b-4; c-3; d-2; e-5
ANSWER
(C) b-4; a-5; c-2; d-1; e-3
EXPLANATION
The bright-field microscope is commonly used to observe stained cells and different stages of cell division. The electron microscope provides extremely high resolution, allowing visualization of viruses that are too small to be resolved by conventional light microscopy. The dark-field microscope is particularly useful for observing thin, unstained, motile organisms such as Leptospira, which appear bright against a dark background. The fluorescence microscope is used to examine fluorescence in situ hybridization (FISH) slides, where fluorescently labelled probes detect specific nucleic acid sequences. The inverted microscope has its objectives positioned below the specimen stage, making it suitable for observing embryos and living cells in culture dishes.