VMC-0039
QUESTION
Which one of the following staining methods is used for fungus demonstration?
OPTIONS
(A) Methylene blue stain
(B) Gram’s stain
(C) Periodic acid-Schiff
(D) Ziehl-Neelsen stain
ANSWER
(C) Periodic acid-Schiff
EXPLANATION
Periodic acid-Schiff (PAS) staining is widely used to demonstrate fungal organisms because it highlights carbohydrate-rich components such as fungal cell walls. Therefore option C is correct; methylene blue and Gram staining may sometimes demonstrate fungi but are not the classic fungal histochemical stain here, while Ziehl-Neelsen is primarily for acid-fast organisms.