Inserted DNA fragments with multiple recognition sequences for restriction endonucleases used for inserting additional DNA by cleavage and ligation are called?

AGB-0028

QUESTION

Inserted DNA fragments with multiple recognition sequences for restriction endonucleases used for inserting additional DNA by cleavage and ligation are called?

OPTIONS

(A) Plasmid
(B) rec DNA
(C) Poly linkers
(D) Chimera

ANSWER

(C) Poly linkers

EXPLANATION

Polylinkers, or multiple cloning sites, are short engineered DNA sequences containing several restriction-enzyme recognition sites and are incorporated into vectors to facilitate insertion of foreign DNA by restriction digestion and ligation. A plasmid is the vector molecule itself, recombinant DNA is DNA formed by joining sequences, and chimera is a broader term for a construct or organism containing genetically distinct components.

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