AGB-0098
QUESTION
Taq polymerase an enzyme used in PCR requires?
OPTIONS
(A) Mg++
(B) Temperature of 72℃
(C) pH 8.4
(D) All of the above
ANSWER
(D) All of the above
EXPLANATION
Taq DNA polymerase requires Mg²⁺ as a cofactor and functions optimally for DNA extension around 72°C; PCR reaction mixtures are also commonly buffered near pH 8.4 under standard conditions. Thus all listed requirements are consistent with the enzyme and standard PCR conditions.
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